D7295
Deoxynucleotide Mix, 10 mM
Molecular Biology Reagent
Synonym(s):
Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
biological source
synthetic (organic)
Quality Level
assay
≥99%
form
liquid
concentration
10 mM
color
colorless
solubility
H2O: soluble at 20 °C
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.
Application
dNTP Mix has been used:
- in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
- in reverse transcription of total RNA to cDNA.
- as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
- routine and long PCR
- manual and automated DNA sequencing
- cDNA synthesis and labeling reactions
Features and Benefits
- Purity of each dNTP: Minimum 99%
- Conveniently formulated; 1 μL is used per 50 μL PCR
- Equimolar amounts of each dNTP means less pipetting
- Minimize risk of contamination in PCR
- UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
了解标准 PCR 方案步骤，查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 8-8 (1989)
Fiona McDonnell et al.
PloS one, 11(4), e0153354-e0153354 (2016-04-29)
Fibrosis and a hypoxic environment are associated with the trabecular meshwork (TM) region in the blinding disease glaucoma. Hypoxia has been shown to alter DNA methylation, an epigenetic mechanism involved in regulating gene expression such as the pro-fibrotic transforming growth
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D7295-.5ML | 04061838104946 |
| D7295-5ML | 04061838104953 |
| D7295-.25ML | 04061838056863 |
| D7295-.2ML | 04061838104939 |
| D7295-20X.2ML | 04061838087355 |