L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with kanamycin sulfate
Synonym(s):
kanamycin agar plates
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About This Item
UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli, selective for coliforms
grade
Molecular Biology
Quality Level
sterility
sterile; autoclaved
form
plates (10 cm), plates (10cm, 20-25ml each)
capacity
20-25 mL
storage temp.
2-8°C
suitability
selective for Escherichia coli, selective for coliforms
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.
Features and Benefits
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Other Notes
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
动物来源培养基
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Articles
基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
Deich RA, et al.
Journal of Bacteriology, 170(2), 489-498 (1988)
Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles
Talhinhas P, et al.
Molecular Biotechnology, 39(1), 57-67 (2008)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L0543-10EA | 04061833948828 |