M4159
Methylene Blue hydrate
suitable for nucleic acid staining, BioReagent
Synonym(s):
3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
EC Number:
200-515-2
Beilstein/REAXYS Number:
3599847
MDL number:
grade
Molecular Biology
Quality Level
product line
BioReagent
form
powder
technique(s)
nucleic acid detection: suitable
solubility
water: 1 mg/mL (dark blue)
suitability
suitable for nucleic acid staining
storage temp.
room temp
SMILES string
O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI
1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChI key
WQVSELLRAGBDLX-UHFFFAOYSA-M
General description
Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.
Application
Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.
Features and Benefits
- Less hazardous than staining with ethidium bromide
- Viewable in visible light
- Fast and easy to use
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Related Content
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| M4159-100G | 04061833077009 |
| M4159-25G | 04061834056829 |