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Merck
CN

MDQ1

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Synonym(s):

DNA Methylation Quantification, DNA methylation shift

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About This Item

NACRES:
NA.54
UNSPSC Code:
41116158
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General description

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Features and Benefits

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Methylated Control DNA, 50 ng/μL
    SDS

pictograms

CorrosionExclamation mark

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Warning

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

wgk

WGK 3

Storage Class

10 - Combustible liquids

Regulatory Information

监管及禁止进口产品

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Protocols

Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。

Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Articles

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.


Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
INSIG2 rs7566605 single nucleotide variant and global DNA methylation index levels are associated with weight loss in a personalized weight reduction program
Pirini F, et al.
Molecular Medicine Reports, 17(1), 1699-1709 (2018)
Vitalina M Komashko et al.
Epigenetics, 5(3), 229-240 (2010-03-23)
Methylation of DNA in combination with histone modifications establishes an epigenetic code that ensures the proper control of gene expression. Although DNA methyltransferases have been shown to interact with histone methyltransferases such as EZH2 (which methylates histone H3 on lysine



Global Trade Item Number

SKUGTIN
MDQ1-48RXN04061835507009
MDQ1-96RXN04061835507016