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WTA2

Complete Whole Transcriptome Amplification Kit

Name: Complete Whole Transcriptome Amplification Kit
Brand: SIGMA

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Synonym(s):

transcriptome amplification kit

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About This Item

NACRES:

NA.55

UNSPSC Code:

41121800

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Properties

Quality Level

200

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

Description

General description

WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library.

Application

Complete Whole Transcriptome Amplification Kit is used for the following applications:

To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.
Reverse transcription and cDNA amplification
For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles
Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)
Amplification of RNA andssDNA viruses for Viral metagenomics from low-input clinical samples

Suitable for use with downstream applications including:

qPCR
microarray analysis
cloning

Biochem/physiol Actions

The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product.

Features and Benefits

Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
Contains all needed components for cDNA amplification
Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism

Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

Library Synthesis Enzyme
SDS

Library Synthesis Solution
SDS

Amplification Mix
SDS

Library Synthesis Buffer
SDS

W4502
Water, Nuclease-Free Water, for Molecular Biology
SDS

Amplification Enzyme
SDS

D7295
Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent
SDS

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

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Protocols

完整全转录组扩增试剂盒实验方案(WTA2)

WTA2（一种全转录组扩增（WTA）方法）能够在区区4小时内，代表性地扩增出纳克级的总RNA而没有3'偏差。

Integration of Sigma^® TransPlex^® with Agilent Microarray

TransPlex^® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Articles

WTA2 Amplicon Modification for NGS

Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Global Trade Item Number

SKU	GTIN
WTA2-10RXN	04061835378814
WTA2-50RXN	04061835378821