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420200

JC-1

Name: JC-1
Brand: MM

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.

Synonym(s):

JC-1, 5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₂₇Cl₄IN₄

CAS Number:

47729-63-5

Molecular Weight:

652.23

MDL number:

MFCD00188475

UNSPSC Code:

12352202

NACRES:

NA.25

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Properties

Quality Level

100

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

red-orange

solubility

DMSO: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

SMILES string

[I-].Clc1cc2[n+](c([n](c2cc1Cl)CC)\C=C\C=[c]3[n](c4c([n]3CC)cc(c(c4)Cl)Cl)CC)CC

InChI

1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

InChI key

FYNNIUVBDKICAX-UHFFFAOYSA-M

Description

General description

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨ_m in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨ_m induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.

Other Notes

Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c