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P2308

Proteinase K from Tritirachium album

Name: Proteinase K from <I>Tritirachium album</I>
Brand: SIAL

lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

Synonym(s):

Endopeptidase K

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About This Item

CAS Number:

39450-01-6

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

254-457-8

NACRES:

NA.54

MDL number:

MFCD00132129

EC Number:

3.4.21.64 (BRENDA, IUBMB)

Specific activity:

≥30 units/mg protein

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Properties

grade

Molecular Biology

product line

BioUltra

form

lyophilized powder

specific activity

≥30 units/mg protein

mol wt

28.93 kDa

composition

Protein, ≥90% biuret

impurities

≤1 ppm DNA

foreign activity

DNAse, Nickase and RNAse, none detected

shipped in

wet ice

storage temp.

−20°C

Description

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Other Notes

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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Articles

Best Product & Supplier for Proteinase K

Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

Guide on How to Use Proteinase K in Different Procedures

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

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Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters.

Mamoru Oshiki et al.

Frontiers in microbiology, 9, 830-830 (2018-05-15)

Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed

m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation.

Ruobing Jia et al.

Molecular cancer, 18(1), 161-161 (2019-11-15)

Dynamic N6-methyladenosine (m6A) RNA modification generated and erased by N6-methyltransferases and demethylases regulates gene expression, alternative splicing and cell fate. Ocular melanoma, comprising uveal melanoma (UM) and conjunctival melanoma (CM), is the most common primary eye tumor in adults and

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Margarita Zayas et al.

PLoS pathogens, 12(1), e1005376-e1005376 (2016-01-05)

Hepatitis C virus (HCV) nonstructural protein (NS)5A is a RNA-binding protein composed of a N-terminal membrane anchor, a structured domain I (DI) and two intrinsically disordered domains (DII and DIII) interacting with viral and cellular proteins. While DI and DII

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Global Trade Item Number

SKU	GTIN
P2308-100MG	04061824819298
P2308-10MG	04061824819311
P2308-25MG	04061824819342
P2308-500MG	04061824819366
P2308-5X1G	04061832701127
P2308-1G	04061824819335
P2308-5MG	04061824819373