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21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

Name: 5(6)-Carboxyfluorescein diacetate <I>N</I>-succinimidyl ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

CFSE

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About This Item

Empirical Formula (Hill Notation):

C₂₉H₁₉NO₁₁

CAS Number:

150347-59-4

Molecular Weight:

557.46

NACRES:

NA.32

PubChem Substance ID:

57647951

UNSPSC Code:

12352108

MDL number:

MFCD00037466

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Properties

Quality Level

200

description

suitable as fluorogenic substrate for esterases

product line

BioReagent

assay

≥90% (HPLC)

form

solid

mol wt

557.46 g/mol

color

almost white to yellow

mp

152-154 °C (lit.)

solubility

DMF: soluble, DMSO: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Description

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Application

5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:

to label cells for flow cytometry
in lymphocyte proliferation assay
to determine the intracellular or intra-organellar pH in chloroplasts
to label Leishmania amazonensis and Leishmania major

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

wgk

WGK 3

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Autophagic induction greatly enhances Leishmania major intracellular survival compared to Leishmania amazonensis in CBA/j-infected macrophages

Dias BRS, et al.

Frontiers in Microbiology, 9 (2018)

Lymphoproliferative responses to dendritic cell presentation of sensitizing allergens in atopic children with multiple allergies

Scott-Taylor TH, et al.

Annals of Allergy, Asthma & Immunology, 119(3), 274-283 (2017)

Generation and characterization of CD1d-specific single-domain antibodies with distinct functional features

Lameris R, et al.

Immunology, 149(1), 111-121 (2016)

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Global Trade Item Number

SKU	GTIN
21888-25MG-F	04061838775702
21888-100MG-F	04061838775696