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B1911

BCIP®/NBT

alkaline phosphatase substrate, chromogenic, liquid

Synonym(s):

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name

BCIP®/NBT Liquid Substrate System, ready to use solution

Quality Level

300

form

liquid

storage temp.

2-8°C

Application

BCIP®/NBT Liquid Substrate System has been used:
  • for staining alkaline phosphatase(ALP) in bone marrow stromal cells
  • as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
  • as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL6183
SLCL0123
SLCJ7143
SLCJ1686
SLCF5540

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Protocols

使用BCIP / NBT底物进行免疫检测

5-溴-4-氯-3-吲哚基磷酸盐（BCIP）/ 硝基蓝四唑（NBT）是一种理想的不溶性底物，可与碱性磷酸酶一起使用。该系统产生蓝紫色产物。可以目视观察到强烈的颜色，非常稳定，并且在暴露于光时不褪色。

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Articles

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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Global Trade Item Number

SKUGTIN
B1911-100ML04061838251626