B5525
Bromophenol Blue sodium salt
Synonym(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
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About This Item
Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
Molecular Weight:
691.94
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
EC Number:
252-170-2
Beilstein/REAXYS Number:
6995701
MDL number:
form
powder
Quality Level
technique(s)
electrophoresis: suitable
solubility
water: soluble
suitability
suitable for nucleic acid electrophoresis
storage temp.
room temp
SMILES string
[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
InChI
1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
InChI key
TWKDJMRHSLWRFI-UHFFFAOYSA-M
General description
Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
Preparation Note
A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B5525-10G | 04061835572076 |
| B5525-25G | 04061835572083 |
| B5525-5G | 04061835572090 |