CMC0016
BL21(DE3) Electrocompetent Cells
Escherichia coli, rod shaped
Synonym(s):
BL21 strain
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About This Item
UNSPSC Code:
41106202
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Product Name
BL21(DE3) Electrocompetent Cells, for protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment
Other Notes
- BL21(DE3) electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
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Protocols
Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.
Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CMC0016-20X25UL | 04061838042439 |
| CMC0016-4X25UL | 04061838042460 |
| CMC0016-20X100UL | 04061838042422 |
| CMC0016-20X50UL | 04061838042446 |
| CMC0016-40X25UL | 04061838042453 |