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D7058

DirectLoad™ Wide Range DNA Marker

ready-to-use marker for DNA electrophoresis

Synonym(s):

agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
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Quality Level

100

form

liquid

usage

100 uses

solubility

water: soluble

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Other Notes

Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCP0373
MKCM8191
MKCG3714
MKCF6588
MKCC2748

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Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

Articles

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Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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Data Sheet - D7058
Bioreagents for DNA/RNA Electrophoresis
Melanie P Dautle et al.
Journal of clinical microbiology, 40(2), 414-421 (2002-02-05)
In this study, 83 clinical isolates purified from biofilms colonizing 18 silicone gastrostomy devices (12 "buttons" and six tubes converted to skin level devices) were selected for subtype characterization utilizing genetic analysis. The tubes, previously used for feeding, remained in

Global Trade Item Number

SKUGTIN
D7058-1VL04061835572724