Select a Size
About This Item
grade
Molecular Biology
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
product line
BioReagent
assay
≥99.9%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
technique(s)
DNA sequencing: suitable, PCR: suitable, transfection: suitable
impurities
≤0.001 meq/g Titratable acid, ≤0.1% water (Karl Fischer)
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
absorption
passes test
suitability
suitable for molecular biology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
General description
- Suitable for Molecular Applications
- Nuclease-Free DMSO
- DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
- Molecular Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Application
- polymerase chain reaction (PCR)
- amplification of cDNA libraries
- DNA sequencing
- column-loading buffers for poly(A)+ RNA selection
- buffers for the transformation of competent E. coli
- transfection protocols.
Dimethyl sulfoxide has been used-
- as an oligonucleotide solvent in array spotting[1]
- as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
- for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
- for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
- in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
Features and Benefits
Disclaimer
Still not finding the right product?
Explore all of our products under Dimethyl sulfoxide
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
Articles
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Related Content
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8418-1L | 04061837896873 |
| D8418-1VL | 04061837299919 |
| D8418-5.5ML | 04061837299926 |
| D8418-500ML | 04054839596322 |
| D8418-1.5ML | 04061837299896 |
| D8418-100ML | 04061837896866 |
| D8418-1ML | 04061837299902 |
| D8418-250ML | 04061833589618 |
| D8418-50ML | 04061837896880 |
| D8418-7.5ML | 04061837299933 |