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D9307

JumpStart™ Taq DNA Polymerase

with MgCl2

Synonym(s):

hot start DNA polymerase, hot start PCR

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.55
MDL number:
MFCD00166026
Concentration:
2.5 units/μL
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Quality Level

200

form

liquid

usage

sufficient for 1500 reactions, sufficient for 250 reactions, sufficient for 50 reactions

feature

dNTPs included: no, hotstart, Hot-Start PCR

concentration

2.5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

JumpStart™ Taq DNA polymerase is a combination of Sigma′s high-performance Taq DNA Polymerase and JumpStart Taq antibody. The Taq DNA Polymerase activity is inactivated by combining the enzyme with JumpStart Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start PCR. During PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature, as the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates, and the polymerase becomes fully active.

Application

  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers
JumpStart™ Taq DNA Polymerase has been used:
  • in the amplification of DNA libraries of varying sizes
  • in a methylation-specific, quantitative real-time polymerase chain reaction (MS-qPCR) to determine the BRCA1 promoter methylation status
  • in the generation of plasmid by amplifying the full-length of HIF1β via PCR

Features and Benefits

  • Reduces non-specific amplification
  • Increases PCR specificity and yield
  • Reduces set-up time concerns associated with manual or wax Hot Start methods
  • Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer containing 15 mM MgCl2

Other Notes

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Regulatory Information

常规特殊物品
低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL4999
SLCM2208
SLCL3987
SLCL3994
SLCK4079

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Protocols

使用Jumpstart™Taq DNA聚合酶(D9307)扩增DNA

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

终点 PCR：特异性和产量增强的抗体介导的热启动 PCR 方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

View All Protocols

Articles

热启动PCR

热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。

简介和历史时间表

了解聚合酶链反应（PCR）的历史，从促进它的发现的基本原理到获得诺贝尔化学奖，以及实时PCR（qPCR）和数字PCR等最近的发展。

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Global Trade Item Number

SKUGTIN
D9307-1SET04061833697115
D9307-50UN04061835572847
D9307-1.5KU04061835572823
D9307-250UN04061835572830
MP0035-1KT04061834066835