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F4135

Fetal Bovine Serum

Name: Fetal Bovine Serum
Brand: SIGMA

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym(s):

HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:

12352207

eCl@ss:

32160409

NACRES:

NA.71

MDL number:

MFCD00132239

Biological source:

bovine fetus

Origin:

USA origin

Sterility:

sterile-filtered

Shipped in:

dry ice

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Properties

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Description

General description

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:

as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
as a component of blocking medium for treating brainstem slices
to improve attachment of dermal explants

Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

Endotoxin and hemoglobin tested

Tested for the presence of bacteria, virus, and mycoplasma
Triple filtered with 0.1 micron membrane under aseptic conditions

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

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Protocols

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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胎牛血清 (FBS) – 基础知识

回顾使用胎牛（或小牛）血清进行细胞培养的基础知识，以及采购和法规如何影响细胞测定所用血清的价格。

用于细胞和组织培养的血清的常见问题解答

查找有关FBS和其他血清产品的常见问题解答(FAQ)以及相关产品的链接。

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Global Trade Item Number

SKU	GTIN
F4135-100ML	04061838255419
F4135-24X500ML	04061833617533
F4135-1L	04061837034718
F4135-500ML	04061838255426
F4135-6X500ML	04061838255433