G1N70
GenElute™ Mammalian Genomic DNA Miniprep Kits
sufficient for 70 purifications
Synonym(s):
Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
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About This Item
usage
sufficient for 70 purifications
Quality Level
technique(s)
DNA purification: suitable
input
mammalian cells
mammalian tissue
test parameters
sample volume: 25 mg tissue, sample volume: 2x10^6 cells
storage temp.
15-25°C
General description
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.
Application
- restriction endonuclease digestions
- PCR
- Southern blots
- sequencing reactions
- cloning
- ligation
Biochem/physiol Actions
Features and Benefits
- Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
- Elution volume: 200 - 400 μl
- Time required: 20 min after lysis
- A260/A280 ratio: 1.6 - 1.9
- Mechanical homogenization required: No
Other Notes
Legal Information
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signalword
Danger
target_organs
Respiratory system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Storage Class
10 - Combustible liquids
Regulatory Information
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Protocols
GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Related Content
DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此，核酸纯化是分子生物学和基因组工作流程中的第一步。
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G1N70-1KT | 04061835573059 |