H7140
Hybridization Solution
for Northern and Southern blotting, liquid (2x concentrate)
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
form
liquid (2x concentrate)
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
General description
Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.
Application
Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.
Preparation Note
Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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Protocols
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Jürgen R Sindermann et al.
American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
Silicon nanonets for biological sensing applications with enhanced optical detection ability
Serre P, et al.
Biosensors And Bioelectronics, 68, 336-342 (2015)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H7140-5X50ML | 04061833803486 |
| H7140-50ML | 04061833803417 |