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L2542

LB Broth (Miller)

Name: LB Broth (Miller)
Brand: SIGMA

Liquid microbial growth medium

Synonym(s):

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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Properties

grade

Molecular Biology

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Description

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
selective cultivation when appropriate antibiotics are added.
the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:

Filter-sterilized, ready-to-use format
Convenient package size
Standard formulation

Other Notes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

动物来源培养基

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Protocols

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Articles

蛋白质表达系统

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Weighing of biomolecules, single cells and single nanoparticles in fluid.

Thomas P Burg et al.

Nature, 446(7139), 1066-1069 (2007-04-27)

Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg = 10(-21) g) have been weighed in vacuum, and proton-level resolution seems to be within reach. Resolving small mass changes requires

Sialylation of group B streptococcal capsular polysaccharide is mediated by cpsK and is required for optimal capsule polymerization and expression.

D O Chaffin et al.

Journal of bacteriology, 187(13), 4615-4626 (2005-06-22)

Several bacterial pathogens have evolved the means to escape immune detection by mimicking host cell surface carbohydrates that are crucial for self/non-self recognition. Sialic acid, a terminal residue on these carbohydrates, inhibits activation of the alternate pathway of complement by

Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50

Antonio Basanta, Beatriz Gomez-Sala, et al.

Biotechnology, 76, 3314-3324 (2010)

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Global Trade Item Number

SKU	GTIN
L2542-500ML	04061833950180