Skip to Content
Merck
CN

Key Documents

View All Documentation

MOD50

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

Synonym(s):

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Preparation Note

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • T3566
    Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
    SDS

signalword

Danger

supp_hazards

EUH031

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Regulatory Information

危险化学品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCD4557
SLCD4559
SLCF7072
SLCG0978
SLCG0975

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Articles

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

Related Content

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
View All Related Papers

Global Trade Item Number

SKUGTIN
MOD50-1KT04061834066804