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NA2110

GenElute™ Bacterial Genomic DNA Kits

sufficient for 70 purifications

Synonym(s):

Bacterial Genomic DNA, bacterial DNA prep, bacterial DNA purification kit, bacterial gDNA extraction kit, bacterial gDNA isolation kit, bacterial gDNA purification kit, bacterial genomic DNA extraction kit, bacterial genomic DNA isolation kit, bacterial genomic DNA purification kit, Gen Elute

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About This Item

NACRES:
NA.55
UNSPSC Code:
41105501
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usage

sufficient for 70 purifications

Quality Level

200

technique(s)

DNA purification: suitable

test parameters

: 70—120 min kit run time, sample volume: 1.5 mL bacterial culture

storage temp.

15-25°C

General description

Click here to view the kit protocol
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Biochem/physiol Actions

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required
  • Typical DNA yields of 15 μg - 20 μg
  • Protocols provided for Gram + and Gram - bacteria
  • High quality genomic DNA in less than 2 hours
  • Purified DNA has an A260/A280 ratio between 1.6 and 1.9

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • P2308
    Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein 3
    SDS
  • C2112
    Column Preparation Solution
    SDS
  • R6148
    RNase A solution
    SDS
  • W0263
    Wash Solution 1
    SDS

signalword

Danger

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

77.0 °F - closed cup

flash_point_c

25 °C - closed cup

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Regulatory Information

常规特殊物品
危险化学品
低风险生物材料

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Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCM1811
SLCL7908
SLCL7909
SLCL7906
SLCH5588

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Protocols

GenElute™细菌基因组DNA试剂盒实验方案（NA2100、NA2110、NA2120）

的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Articles

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Related Content

DNA纯化_RNA提取_核酸纯化-默克生命科学

DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此，核酸纯化是分子生物学和基因组工作流程中的第一步。

DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

User Guide - NA2100, NA2110, NA2120
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Global Trade Item Number

SKUGTIN
NA2110-1KT04061835573745