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About This Item
NACRES:
NA.55
UNSPSC Code:
41105501
usage
sufficient for 350 purifications
technique(s)
DNA purification: suitable
test parameters
: 70—120 min kit run time, sample volume: 1.5 mL bacterial culture
storage temp.
15-25°C
General description
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
The purified bacterial genomic DNA is ready for downstream applications such as:
GenElute™ Bacterial Genomic DNA Kits has been used:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
GenElute™ Bacterial Genomic DNA Kits has been used:
- to isolate DNA
- to prepare DNA from E. coli strain
- to extract genomic DNA
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
Biochem/physiol Actions
The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Features and Benefits
- Typical DNA yields of 15 μg - 20 μg
- Protocols provided for Gram + and Gram - bacteria
- High quality genomic DNA in less than 2 hours
- Purified DNA has an A260/A280 ratio between 1.6 and 1.9
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
77.0 °F - closed cup
flash_point_c
25 °C - closed cup
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Regulatory Information
低风险生物材料
危险化学品
常规特殊物品
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Protocols
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era
VZemlivckova H, et al.
Journal of Medical Microbiology, 67(7), 1003-1003 (2018)
Modulation of Global Transcriptional Regulatory Networks as a Strategy for Increasing Kanamycin Resistance of the Translational Elongation Factor-G Mutants in Escherichia coli
Mogre A, et al.
G3 (Bethesda, Md.), g3-300284 (2017)
Ikrema Hassan et al.
Genome announcements, 4(1) (2016-01-16)
Here we report the complete genomic sequence of the bacterium Arthrobacter sp. strain LS16, consisting of a single circular chromosome of 3.85 Mb with no identified plasmid. Data contained within will facilitate future genetic modification and engineering of the Arthrobacter sp.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| NA2120-1KT | 04061834119104 |