Phosphatase, Alkaline from calf intestine

Name: Phosphatase, Alkaline from calf intestine
Brand: SIGMA

buffered aqueous glycerol solution

Synonym(s):

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

eCl@ss:

42010105

NACRES:

NA.53

EC Number:

3.1.3.1 (BRENDA, IUBMB)

MDL number:

MFCD00131849

Concentration:

≥10,000 units/mL

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Properties

grade

Molecular Biology

form

buffered aqueous glycerol solution

mol wt

~80 kDa

concentration

≥10,000 units/mL

UniProt accession no.

P19111

foreign activity

DNase, RNase, none detected

shipped in

wet ice

storage temp.

−20°C

Gene Information

cow ... ALPI(280993)

Description

General description

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Application

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Other Notes

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl₂, and 0.1 mM ZnCl₂, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.