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Merck
CN

R7644

Transcript RNA Markers 0.28-6.6 kb

for RNA electrophoresis

Synonym(s):

0.28-6.6 kb Transcript RNA Markers, Transcript RNA markers for RNA electrophoresis

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105337
Form:
liquid
Storage temp.:
−70°C
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grade

Molecular Biology

Quality Level

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

General description

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) contains 9 RNA transcripts from 281 – 6,583 bases. Three μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Transcript RNAMarkers 0.28-6.6 kb are suitable for use as an RNA ladder for formaldehyde agarose gel electrophoresis. It is also suitable for the size determination of small RNA using native or denaturing agarose gel electrophoresis.

Other Notes

Contains a mixture of nine RNA transcripts, 281–6,583 bases
For optimal resolution, the recommended agarose gel concentration is 1.0%.
Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

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Protocols

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Articles

了解 mRNA 疫苗如何诱导免疫,以及如何为疫苗免疫原和其他生物制药制备合成 mRNA。寻找合成 mRNA 所需的试剂。

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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Related Content

用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具,用于基于 mRNA 的疫苗和疗法开发。

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

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Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization.
Pinaud R, et al.
Nature Protocols, 3, 1370-1379 (2008)



Global Trade Item Number

SKUGTIN
R7644-1VL04061836696597