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T3449

TrypZean® Solution, 1×

recombinant, expressed in corn, sterile-filtered

Synonym(s):

Trypsin

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352204
Recombinant:
expressed in corn
Concentration:
1 ×
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recombinant

expressed in corn

Quality Level

300

sterility

sterile-filtered

form

aqueous solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

7.70-8.30

shipped in

dry ice

storage temp.

−20°C

General description

TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Application

TrypZean® Solution, 1× has been used:

  • for the suspension of pellet during DNA extraction
  • digestion of cells to single-cell suspension for pharmacotyping of 2D cells
  • as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium

Features and Benefits

TrypZean® Solution provides the following features and benefits:
  • Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
  • TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
  • Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
  • High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
  • Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

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Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBL8259
RNBL4895
RNBL3434
RNBK7718
RNBK4189

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Protocols

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Cancer Stem Cell Tumorsphere Formation Protocol

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

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Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

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Global Trade Item Number

SKUGTIN
T3449-100ML04061837344480
T3449-500ML04061837344497