基因表达和基因沉默
调节基因表达是对控制生物过程的复杂遗传网络进行研究的研究人员所采用的最强大技术之一。基因调节实验尤其被广泛用于确定疾病治疗方案的有效性，包括从癌症到发育和神经退行性疾病等。
目前有大量的技术可用于有效调节细胞和组织中的基因表达。RNAi（RNA干扰）是一种自然的生物学机制，其中siRNA（小干扰RNA）双链体可诱导有效的靶向基因表达抑制。在面向研究人员开发商用RNAi 产品方面，SigmaAldrich® Advanced Genomics一直走在前沿。
最近，CRISPR抑制（CRISPRi）和CRISPR激活（CRISPRa）的发展为研究人员提供了一套强大的新工具，让几乎无限的基因调控选项成为可能。利用SigmaAldrich® Advanced Genomics非凡的CRISPR产品，研究人员几乎可准确有效地敲低、敲除、激活或调高任何基因。
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Diagram showing the mechanistic flow of shRNA and siRNA mediated gene silencing in cells.
RNAi - 基因调控照亮遗传路径
功能基因组学筛选是分子靶点发现研究必不可少的环节，科学家可依靠该技术揭示生物系统、通路和疾病背后的潜在机制。对这些复杂的分子互作网络的有效研究可通过使用shRNA文库进行可靠、高效和长期的基因敲减来实现。
Sigma-Aldrich® Advanced Genomics是RNAi协会TRC1.5和TRC2集合的唯一指定供应商。这类慢病毒shRNA文库基因组覆盖率全面，提供包括全基因组、单独RNAi克隆/载体和基因家族集的多种产品形式。
CRISPRi和CRISPRa - 强大的基因抑制和激活
CRISPRi（CRISPR干扰）和CRISPRa（CRISPR激活）可在不改变潜在的DNA序列的情况下分别提供高效的基因沉默和激活。当用于大规模 LOF（功能丧失）和 GOF（功能获得）筛选中时，研究人员能够识别出其他方法经常遗漏的独特但功能相关的基因通路。
带有协同激活介导因子复合物（SAM）的CRISPRa和使用KRAB域的CRISPRi的示意图。
Sigma-Aldrich® Advanced Genomics提供了一整套用于基因敲减和过表达实验的优化CRISPRi和CRISPRa文库目前可提供CRISPRi和SAM CRISPRa混合的慢病毒文库，也可根据您的特定需求提供定制产品。
担心CRISPR不适合您的实验项目？连线咨询Sigma-Aldrich®Advanced Genomics专家。
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