酶联免疫吸附测定(ELISA)
ELISA通常在多孔微孔板中进行，属于分子生物学测定法，常用于多肽、蛋白和抗体等各种分子的检测和定量。这种测定方法可检测pg/mL水平的目标分子，同时在基础研究和疾病研究中发挥着重要的作用。
ELISA检测法：抗原-抗体相互作用
ELISA的基本分子成分通常包括酶偶联抗体、固定的目标分子和检测底物。ELISA获取数据的成功率和质量取决于抗体-抗原相互作用的亲和性和特异性，受多种因素影响，包括pH、温度和离子强度等。
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探索如何在Luminex®仪器上可靠地开展MILLIPLEX®多重免疫测定，在确保稳健测定性能的同时不断扩展产品组合以满足您的各种生物标记物检测需求。
ELISA检测法
直接法ELISA先使用直接结合酶标板或间接结合捕获抗体的固定抗原，再使用抗原特异性酶联一抗和检测底物。
更常用的间接检测法先使用未偶联一抗，再使用特异性检测一抗的酶联二抗。间接法只有二抗与酶偶联，因而对目标抗原的免疫反应性更强。
直接和间接检测法之外，捕获或“夹心”检测法先使用额外的抗原捕获抗体结合微孔板表面，然后类似上述间接法使用一抗和酶联二抗。
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