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03-0163-00

SMC® 人TNFα 高灵敏度试剂盒

Used for ultrasensitive quantification of Human TNFα in human serum and plasma

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肿瘤坏死因子 α

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NACRES:
NA.77
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
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产品名称

SMC® 人TNFα 高灵敏度试剂盒,

description

Used for ultrasensitive quantification of Human TNFα in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

P01375

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

SMC® 人TNFα高灵敏度免疫分析试剂盒包含进行分析所需的所有试剂。该测定采用以标准孔板形式配置的标准免疫测定工作流程。

测定方案与现有的夹心ELISA方法相似，但有两个主要区别：
1）洗脱缓冲液破坏夹心结构，分离标记的检测抗体以进行定量。
2）Erenna 和SMCxPRO® 免疫测定系统使用单分子计数（SMC®）技术对分析物进行检测。

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
  • Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
  • Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
  • Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 50-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 113% Plasma: 105%
  • Recovery: Serum: 95% Plasma: 83%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although TNFα was detectable in non-human primate, rat, feline, canine and equine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

我们的产品仅用于研究目的，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用对人类或动物。

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

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全球贸易项目编号

货号GTIN
03-0163-0004054839591730