Quality Level
form
crystalline powder
usage
sufficient for 50 reactions
feature
dNTPs included: no, hotstart: no
concentration
20 units/μL
technique(s)
RT-PCR: suitable, cDNA synthesis: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
eAMV™ 逆转录酶是一种增强型禽成髓细胞病病毒（AMV）逆转录酶，负责合成与 RNA、DNA 或 RNA:DNA 杂交体互补的 DNA 链。这种异常强大的 AMV逆转录酶比标准 AMV 或 Moloney 鼠白血病病毒（M-MLV）逆转录酶具有更优的热稳定性。eAMV™ RT 是从总 RNA 或 poly（A）+RNA获得高质量全长 cDNA 的理想酶，并且在转录长靶标方面也是有效的。
Application
Enhanced Avian Reverse Transcriptase可将RNA转录为DNA，同时显著提升mRNA模板合成cDNA的效率。这是由于本款增强型AMV-RT能够逆转录长链mRNA模板，能够有效穿越复杂的二级结构，可以通过RT-PCR反应检测低丰度mRNA。
增强型禽逆转录酶[eAMV™逆转录酶]已用于总 RNA 的逆转录，以在定量逆转录 PCR（RT-qPCR）分析中合成 cDNA。
增强型禽（eAMV）逆转录酶是一种高度纯化的禽成髓细胞瘤病毒逆转录酶（AMV-RT），与标准AMV-RT或标准莫洛尼鼠白血病病毒逆转录酶（MMLV-RT）相比，具有更高的性能。
这种异常的稳健AMV-RT具有增强的检测低丰度信息，在高温（高达65℃）下通过困难的二级结构转录以及转录高达14.1 kb的mRNA模板的能力。
这种异常的稳健AMV-RT具有增强的检测低丰度信息，在高温（高达65℃）下通过困难的二级结构转录以及转录高达14.1 kb的mRNA模板的能力。
Biochem/physiol Actions
逆转录酶能够加速dNTP形成磷酸二酯键，从而催化RNA模板整合到互补DNA中。
Features and Benefits
- 对低丰度信使核糖核酸的敏感性更高
- 在高温（高达 65°C）下通过复杂二级结构进行的无核转录
- 高效生成高达 14.1kb的全长 cDNA
- 产生可用于 PCR 扩增的第一链 cDNA
Packaging
提供1管10x反应缓冲液。
Other Notes
一单位酶能够以多聚腺苷酸为模板，以12-18的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA沉淀材料中。
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
商品
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
相关内容
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 5-5 (1989)
C Tosh et al.
Acta virologica, 41(3), 153-155 (1997-06-01)
A method of reverse transcription (RT) and polymerase chain reaction (PCR) amplification of 1D (VP1) gene of foot-and-mouth disease (FMD) virus using one reaction mixture containing both avian myeloblastosis virus (AMV) reverse transcriptase (RTase) and Tfl DNA polymerase is described.
K Dukas et al.
Analytical biochemistry, 215(1), 66-72 (1993-11-15)
We developed a reverse transcription-polymerase chain reaction (RT-PCR) method which permits the simultaneous amplification of several mRNAs, even though their relative levels may be very different. First-strand cDNAs were synthesized from total RNA by MMLV reverse transcriptase with oligo(dT)15 priming.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A4464-1KU | 04061833374504 |