C3868
Anti-Calsequestrin-2 兔抗
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-CASQ2, Anti-fast-twitch, cardiac muscle Calsequestrin
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关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
10
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~55 kDa
species reactivity
rat, human
species reactivity (predicted by homology)
mouse
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2, western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CASQ2(845)
mouse ... Casq2(12373)
rat ... Casq2(29209)
General description
Calsequestrin is a major Ca2+ binding protein in cardiac and skeletal muscle.
Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.
Immunogen
synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.
Application
Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.
Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)
Biochem/physiol Actions
Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the sarcoplasmic reticulum (SR).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
Calsequestrin 2 and arrhythmias
Faggioni M and Knollmann B C.
American Journal of Physiology. Heart and Circulatory Physiology, 302(6), H1250-H1260 (2011)
D H MacLennan et al.
Proceedings of the National Academy of Sciences of the United States of America, 68(6), 1231-1235 (1971-06-01)
An acidic protein has been extracted from sarcoplasmic reticulum with KCl and deoxycholate. The protein, which remains soluble after extraction, has been highly purified by fractionation on DEAE-cellulose, Sephadex, and hydroxylaptite. It has a molecular weight of 44,000 and contains
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio
Furlan S, et al.
Journal of Muscle Research and Cell Motility, 37(1-2), 27-39 (2016)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C3868-200UL | 04061838030405 |