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磷酸钙转染试剂盒

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

别名:

Gene delivery

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关于此项目

UNSPSC Code:
12352200
EC Number:
233-140-8
NACRES:
NA.85
Form:
solution
Grade:
Molecular Biology
Technique(s):
transfection: suitable
技术服务
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grade

Molecular Biology

form

solution

usage

 kit sufficient for 160 transfections (6 cm dishes),  kit sufficient for 400 transfections (3.5 cm dishes),  kit sufficient for 80 transfections (10 cm dishes)

technique(s)

transfection: suitable

shipped in

dry ice

storage temp.

−20°C

General description

磷酸钙法转染是将DNA引入真核细胞的常用方法。这一技术已经在广泛的细胞类型中使用,可实现瞬时和稳定的转染效果。

Application

磷酸钙转染试剂盒用于:
  • 启动转染
  • 转染 Hek293T 细胞
  • 转染 H29D 细胞

适用于瞬时和稳定地将DNA转染至培养的哺乳动物细胞中。 使用磷酸钙方法已成功转染了以下细胞:

BAEC
肠黑素瘤细胞
CHO K1
COS-7
成纤维细胞(人胚胎、新皮肤)
HEK293
Huh7
IMR-90
LLC(Lewis肺癌)
NIH3T3
PC-12
PCI-13
SH-SY5Y
SK-HEP-1
T47D

Biochem/physiol Actions

该方法是基于将含有磷酸钠的HEPES缓冲盐水和含有DNA的CaCl2 溶液进行缓慢混合实现的。 所形成的DNA-磷酸钙共沉淀物可粘附于细胞表面,并可能通过胞吞作用被细胞吸收。

Features and Benefits

  • 适用于瞬时和稳定转染
  • 可重复用于多种细胞类型
  • 广泛引用
  • 价格便宜

Other Notes

磷酸钙转染试剂盒包含:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES 缓冲盐 (H1012)
25 ml 分子生物学级纯水(W4502)
该方案可用于使用pSV40-CAT质粒作为报告基因对CHO细胞进行瞬时转染。


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

商品

转染是将DNA、RNA或蛋白质引入真核细胞的过程,用于研究和调节基因表达。因此,转染技术和实验方案作为分析工具,有助于表征遗传功能、蛋白质合成、细胞生长和发育。

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

相关内容

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.


A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
A Calcium Phosphate Transfection
Ausubel, F. et al (ed.)
Short Protocols in Molecular Biology, 3rd, 9-9 (1995)
Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in



全球贸易项目编号

货号GTIN
CAPHOS-1KT04061833531464