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CE0050

CelLytic ™ MEM 蛋白提取试剂盒

别名:

Membrane protein extraction kit

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关于此项目

NACRES:
NA.56
UNSPSC Code:
41116134
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technique(s)

protein extraction: suitable

Quality Level

200

shipped in

wet ice

storage temp.

−20°C

General description

CelLytic™ MEM蛋白提取试剂盒提供了一种分离细胞中疏水性筏状微结构域相关蛋白的快速、方便的方法。该方法基于相分离，不需要分离细胞膜。分离的蛋白质可用于进一步的实验，例如 SDS-PAGE、蛋白质印迹、斑点印迹和免疫沉淀。 该试剂盒已在 HeLa、HEK-293、NIH 3T3、COS 和 CHO 及其它细胞系上进行了测试。
膜蛋白占据了生物体基因组大约20-30%的成分，充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能，例如将细胞与外部的毒素隔离开来，作为胞内信号级联的起始点，以及维持临界离子浓度。

Application

CelLytic™ MEM 蛋白质提取试剂盒已被用于以肝细胞测定脂肪从头 合成和提取用于蛋白质印迹的膜蛋白。
提供足够进行80次检测的试剂。

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Lysis and Separation Buffer 50 mL
  • Wash Buffer for CelLytic MEM 50 mL
  • Sodium Chloride, 4M Solution 1.5 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书 & 价格
  • P8340
    Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
    化学品安全说明书

pictograms

Exclamation mark
GHS07

存储类别

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 2

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2

法规信息

常规特殊物品
低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
108M4183V
11180515
107M4124V
11170528
086M4103V

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Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to
Yue Zhao et al.
Cancer medicine, 7(8), 3977-3987 (2018-07-06)
Esophageal squamous cell carcinoma (ESCC) is a malignant disease with poor prognosis. Because of early metastasis prior to diagnosis and therapeutic resistance, ESCC has become one of the leading causes of cancer-related death. Here, we investigated the clinicopathological significance of
Wei He et al.
Annals of translational medicine, 10(4), 218-218 (2022-03-15)
Colonic mucosal injuries are an important manifestation of ulcerative colitis (UC), which is related to hypoxia-induced glycolysis in colonic mucosal epithelial cells (cmECs). Panax notoginseng (PN) promotes the repair of colonic mucosal injuries by inhibiting hypoxia-induced glycolysis in cmECs; However
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全球贸易项目编号

货号GTIN
CE0050-1KT04061835518050