form
liquid
usage
100 uses (0.1 mg size), 250 uses (250 μg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
Application
123 bp DNA 分子量标准已被用作随机扩增多态性 DNA-聚合酶链式反应 (PCR) 分析中的大小标记。它也被用作凝胶电泳的分子量标准。
Other Notes
Sigma 的 123 bp DNA 分子量标准是在 10 mM Tris-HCl (pH 7.5)、50 mM NaCl 和 0.1 mM EDTA 溶液中提供的。
为实现最佳分辨率，建议采用的琼脂糖凝胶浓度为1.5%。
包含35个片段，123-4182 bp
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
此项目有
商品
选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.
J L Hartley et al.
Gene, 13(4), 347-353 (1981-05-01)
A method for self-ligation of DNA segments, which is based on the rotational non-equivalence of ends produced by AvaI cleavage, has been developed. Using this method and an initiator molecule to encourage the formation of long polymers, we have obtained
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D5042-.1MG | 04061838255181 |
| D5042-100UG | 04055977220308 |
| D5042-250UG | 04061835572717 |