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D5671

DMEM - high glucose

With sodium Bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
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产品名称

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: high
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

这种DMEM高葡萄糖培养基是一种不含添加因子（常见修改）如L-丙氨酰-L-谷氨酰胺、HEPES或丙酮酸钠的1x完全培养基。 它与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。 该培养基需要添加L-谷氨酰胺或于L-丙氨酰-L-谷氨酰胺。

Application

Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

Preparation Note

添加0.584 g/L L-谷氨酰胺。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0550
RNBM0069
RNBL8843
RNBL7075
RNBL6563

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实验方案

四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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全球贸易项目编号

货号GTIN
D5671-1L04061833586587
D5671-20L04061837340437
D5671-24X500ML04061833586594
D5671-10L04061837340420
D5671-500ML04061833586600
D5671-6X1L04061833586617
D5671-6X500ML04061833586624