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Merck
CN

D6920

2′-脱氧腺苷5′-三磷酸 钠盐 溶液

10 mM

别名:

dATP

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关于此项目

NACRES:
NA.52
UNSPSC Code:
41106305
EC Number:
217-662-3
Assay:
≥99%
Form:
liquid
Storage temp.:
−20°C
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assay

≥99%

Quality Level

form

liquid

concentration

10 mM

technique(s)

PCR: suitable

color

colorless

foreign activity

DNase, RNase, NICKase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

General description

2′-脱氧腺苷5′-三磷酸(dATP)由脱氧核糖上的一个碱基和糖上与一个由三个磷酸残基组成的链相结合的糖上的5′-羟基组成。细胞利用dATP通过DNA聚合酶合成DNA。

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution has been used as a component of the dNTP mix for labelling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe.
2′-脱氧腺苷5′-三磷酸盐钠盐可用于DNA合成反应,如PCR、DNA测序和分子克隆技术。


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存储类别

11 - Combustible Solids

wgk

WGK 3



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

相关内容


Spittlebugs (Hemiptera)
Kuznetsova V G, et al.
Protocols for Cytogenetic Mapping of Arthropod Genomes., 351-380 (2015)
Christina M Zimanyi et al.
Structure (London, England : 1993), 20(8), 1374-1383 (2012-06-26)
Ribonucleotide reductases (RNRs) provide the precursors for DNA biosynthesis and repair and are successful targets for anticancer drugs such as clofarabine and gemcitabine. Recently, we reported that dATP inhibits E. coli class Ia RNR by driving formation of RNR subunits
Kousuke Sato et al.
Chembiochem : a European journal of chemical biology, 12(15), 2341-2346 (2011-09-03)
We herein describe the synthesis of fluorescent 5-(5,6-dimethoxybenzothiazol-2-yl)-2'-deoxyuridine 5'-triphosphate (d(bt)UTP) and primer extension reactions using d(bt)UTP. We also carried out primer extension reactions using the (bt)U template. B family DNA polymerases, such as KOD, Deep Vent (exo-), and 9°N(m) DNA



全球贸易项目编号

货号GTIN
D6920-.5ML04061838214461