D9156
脱氧核糖核酸，单链 来源于鲑鱼睾丸
For hybridization
别名:
single-stranded template DNA
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关于此项目
Assay:
9-11 mg/mL (DNA concentration)
Biological source:
fish testis (salmon)
Form:
solution
Storage temp.:
−20°C
biological source
fish testis (salmon)
Quality Level
grade
Molecular Biology
assay
9-11 mg/mL (DNA concentration)
form
solution
concentration
10 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
General description
是从鲑鱼睾丸分离优质单链模板DNA的即用型解决方案。 溶液的供应浓度为9-12 mg/ml。
Application
许多因素有助于提高核酸杂交的信噪比。 这些因素包括溶剂（甲酰胺）的存在、杂交温度、杂交长度、杂交溶液体积、搅拌程度和方法、封闭剂的使用、探针浓度和比活性、使用分子试剂提高核酸重缔合的速率以及在洗涤膜的严格程度。
为了减少探针与基质的任何非特异性杂交，必须使用封闭剂。 通常，使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA，可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
为了减少探针与基质的任何非特异性杂交，必须使用封闭剂。 通常，使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA，可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
适合用作Northern和Southern印迹的封闭剂。
鲑鱼睾丸的单链脱氧核糖核酸适合用作Southern杂交中的封闭剂。用于骨髓活检样本 和人血单核细胞亚群的原位杂交组织化学研究。
Preparation Note
将该DNA用乙醇沉淀并超声处理，产生单链片段，其与587和831碱基对标记片段共迁移。
Other Notes
DNA溶液要在室温下放置进行再退火，因此建议使用前将溶液煮沸10分钟，然后在冰上至少冷却5分钟。
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
商品
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
Emilia Huerta-Sánchez et al.
Nature, 512(7513), 194-197 (2014-07-22)
As modern humans migrated out of Africa, they encountered many new environmental conditions, including greater temperature extremes, different pathogens and higher altitudes. These diverse environments are likely to have acted as agents of natural selection and to have led to
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D9156-1ML | 04061833590355 |
| D9156-5ML | 04061833590362 |
| D9156-5X1ML | 04061833590379 |