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Merck
CN

F4799

3xFLAG Peptide

≥90% (HPLC/MS), lyophilized powder

别名:

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk肽, dykddddk肽

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关于此项目

经验公式(希尔记法):
C120H169N31O49S
分子量:
2861.87
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
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产品名称

3X FLAG® 肽, lyophilized powder

assay

≥90% (HPLC/MS)

Quality Level

form

lyophilized powder

shipped in

wet ice

storage temp.

2-8°C

General description

3X FLAG肽是一种具有23个氨基酸残基的合成肽。在该肽中Asp-Tyr-Lys-Xaa-Xaa-Asp基序重复了三次。在C-末端的八个氨基酸组成了经典的FLAG序列(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)。
将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上洗脱时,建议工作浓度为100 μg/ml。

Application

用于从在溶液中的或结合至抗-FLAG® M2琼脂糖亲和凝胶(A2220)上琼脂糖上的抗-FLAG ®M2单克隆抗体(F1804)进行3X FLAG®融合蛋白的竞争性洗脱。通过亲和色谱法可实现这一目标。用于将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上温和的洗脱。1X FLAG肽(F3290)将不会把3X FLAG融合蛋白从抗-FLAG®亲和凝胶上洗脱下来。


请访问我们的FLAG® 应用 门户网站,了解更多产品详情。

Preparation Note

如需制备储备溶液,可溶解于TBS(含有150 mM NaCl的50mM Tris-HCl,pH7.4)至5 mg/mL的浓度。

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

免疫沉淀(IP)可用于高效、高产量分离和纯化与FLAG®肽标签融合的蛋白质。此过程采用ANTI-FLAG® M2亲和凝胶进行,后者是一种与琼脂糖树脂共价结合的高度特异性单克隆抗体。

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

商品

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

相关内容

默克分享基因组学、克隆学和大量分子生物学技术的发展使研究人员能在众多生物系统中表达异源蛋白。表达重组蛋白的能力为研究人员提供了广泛而强大的下游应用空间,以便进一步开展研究。

用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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全球贸易项目编号

货号GTIN
F4799-1MG04061824807516
F4799-4MG04061833617908
F4799-25MG04061833617892