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关于此项目
Biological source:
bovine pituitary glands
Assay:
~90% (SDS-PAGE and N-terminal analysis)
Form:
lyophilized powder
Mol wt:
16.4 kDa
Impurities:
<0.5 EU/vial
产品名称
Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture
biological source
bovine pituitary glands
Quality Level
assay
~90% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
0.03-3.0 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
16.4 kDa
packaging
pkg of 1 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<0.5 EU/vial
color
white
UniProt accession no.
storage temp.
−20°C
Gene Information
bovine ... FGF2(281161)
General description
The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.
Application
A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:
Fibroblast Growth Factor-Basic from bovine pituitary has been used:
- In animal and human cell culture
- In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
- In the generation of directly reprogramming fibroblast
- In human glioma cell culture
Biochem/physiol Actions
Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.
Physical form
Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin
Preparation Note
Purified glands by a modification of the method of Gospodarowicz, et al.
Analysis Note
The proliferative activity is tested in culture using fetal bovine heart endothelial cells.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs
Capkin M, et al.
Biomedical Materials (Bristol, England), 7(4), 045013-045013 (2012)
Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs
Capkin M, et al.
Biomedical Materials (Bristol, England), 7(4), 45013-45013 (2012)
Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran
Kazemiha VM, et al.
Cytotechnology, 66(5), 861-873 (2014)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| F5392-1UG | 04061833618202 |