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Merck
CN

GE17-0618-01

Protein G Sepharose 4 Fast Flow

Cytiva 17-0618-01, pack of 5 mL

别名:

Fast Flow resin, Antibody purification resin, IgG purification resin

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关于此项目

NACRES:
NA.56
UNSPSC Code:
41106500
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form

resin

crosslinking

cross-linked

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-0618-01

storage condition

(20% Ethanol)

parameter

<0.1 Mpa pressure, 150-250 cm/hr flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

4% cross-linked agarose

matrix active group

Recombinant streptococcal protein G lacking the albumin-binding region, produced in E. coli

average diameter

90 μm

cleaning in place

2-10

working range

3-9

capacity

≥20 mg binding capacity(human IgG/mL resin)

separation technique

affinity

storage temp.

2-8°C

General description

Protein G Sepharose 4 Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow.

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

As member of the BioProcess media range, Protein G Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Binding specificities that complement Protein A media.
  • Binds a broad range of IgG species and subclasses.
  • Multi-point attachment minimizes ligand leakage.
  • Used in a range of research applications.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva


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存储类别

3 - Flammable liquids

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历史批次信息供参考:

分析证书(COA)

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实验方案

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

商品

实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

本页介绍了高效的色谱柱填充和抗体亲和层析的制备。

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

查看所有文章

Ting-Ting Du et al.
Nature communications, 10(1), 1117-1117 (2019-03-10)
Sensory hair cells, the mechanoreceptors of the auditory and vestibular systems, harbor two specialized elaborations of the apical surface, the hair bundle and the cuticular plate. In contrast to the extensively studied mechanosensory hair bundle, the cuticular plate is not
Anthony Rössl et al.
The Journal of biological chemistry, 294(16), 6227-6239 (2019-02-26)
Gcn5 and sirtuins are highly conserved histone acetyltransferase (HAT) and histone deacetylase (HDAC) enzymes that were first characterized as regulators of gene expression. Although histone tails are important substrates of these enzymes, they also target many nonhistone proteins that function
Ting-Ting Du et al.
Nature communications, 10(1), 1117-1117 (2019-03-10)
Sensory hair cells, the mechanoreceptors of the auditory and vestibular systems, harbor two specialized elaborations of the apical surface, the hair bundle and the cuticular plate. In contrast to the extensively studied mechanosensory hair bundle, the cuticular plate is not



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货号GTIN
GE17-0618-0104061837620522