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GE17-0033-01

Sephadex® G-25 Medium

Cytiva 17-0033-01, pack of 100 g

别名:

Gel Filtration Resin

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NACRES:
NA.56
UNSPSC Code:
41115722
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form

resin (dry)

crosslinking

cross-linked

mol wt

protein Mr 800-5000 Da (fractionation range), protein Mr 8000 Da (exclusion limit)

packaging

pack of 100 g

manufacturer/tradename

Cytiva 17-0033-01

storage condition

dry media

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked dextran

particle size

50-150 μm (dry), 85-255 μm (wet)

cleaning in place

2-13

operating pH

2-13

separation technique

size exclusion (SEC)

storage temp.

2-30°C

General description

Sephadex® G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
  • BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

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存储类别

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

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实验方案

亲和层析缓冲液置换和脱盐

如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。

使用Sephadex®进行分离

本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

相关内容

Separation Options with Sephadex®

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

Sephadex® and Darcy’s Law

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

全球贸易项目编号

货号GTIN
GE17-0033-0104061834083344