H1404
肝细胞生长因子 人
>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture
别名:
HGF, 散射因子, 肝素A
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Biological source:
human
Recombinant:
expressed in Baculovirus infected High-5 cells
Assay:
>95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~80 kDa
Impurities:
<1.0 EU/μg
产品名称
肝细胞生长因子 人, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in Baculovirus infected High-5 cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
potency
1.0-20.0 ng/mL
quality
endotoxin tested
mol wt
~80 kDa
packaging
pkg of 5x5 μg, pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1.0 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... HGF(3082)
Biochem/physiol Actions
肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。
Physical form
从经0.2μm过滤的含有20 mM NaH2PO4 (pH 7.0)和500 mM NaCl,并且每1μg HGF中含50μg BSA的溶液冻干得到
Preparation Note
在含有至少0.1％人或牛血清白蛋白的无菌PBS中以50μg/mL的浓度进行复溶。复溶后，该溶液可以在2-8°C下最多保存一个月，或在-20°C到-70°C下最多保存三个月。请使用手动除霜冷冻柜，并避免反复冻融。
Analysis Note
生物学活性通过其诱导Saos-2人骨肉瘤细胞分泌IL-11的能力进行测定。
Other Notes
NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
新产品
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商品
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
Masaki Kobayashi et al.
Biochemical and biophysical research communications, 527(1), 153-161 (2020-05-25)
MicroRNA (miRNA) plays a pivotal role in cancer biology. Therefore, tumor suppressor (TS) miRNAs are an attractive target for cancer therapy. However, clinical trials have failed due to the difficulties in miRNA delivery, warranting the development of a novel drug
Ryuichi Tatsumi et al.
American journal of physiology. Cell physiology, 297(2), C238-C252 (2009-06-12)
Regenerative coordination and remodeling of the intramuscular motoneuron network and neuromuscular connections are critical for restoring skeletal muscle function and physiological properties. The regulatory mechanisms of such coordination remain unclear, although both attractive and repulsive axon guidance molecules may be
R A Furlong
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(9), 613-617 (1992-09-01)
Hepatocyte growth factor, a potent mitogen for epithelial and other cell types, and scatter factor, a stimulant of epithelial cell motility are identical. In addition to these mitogenic and motogenic functions, the factor has been shown to be an epithelial
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H1404-5UG | 04061833665237 |
| H1404-5X5UG | 04061833665251 |