跳转至内容
Merck
CN

主要文件

查看所有文档

H3506

透明质酸酶 来源于牛睾丸

Type I-S, lyophilized powder, 400-1000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

化学文摘社编号:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC 号:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
400-1000 units/mg solid
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

type

Type I-S

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

UniProt accession no.

Q5E985, Q8SQG8

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Application

来自牛睾丸的透明质酸酶已用于乳腺组织、心脏组织和卵母细胞的消化以用于细胞的分离。

适用于制备用于测序的单细胞悬液。

Biochem/physiol Actions

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

Other Notes

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
更多关于用于复合碳水化合物分析的酶的详细信息请见www.sigma-aldrich.com/enzymeexplorer

Still not finding the right product?

Explore all of our products under 透明质酸酶 来源于牛睾丸

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCH0779
SLCF5379
SLCD3593
SLCC2160
SLBR7365V

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

实验方案

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

商品

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

相关内容

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Complex Carbohydrate Analysis- Enzymes, Kits and Reagents
Product Information sheet - H3506
Heikki J Nieminen et al.
Scientific reports, 7(1), 3991-3991 (2017-06-24)
Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound
Sarah A Flowers et al.
Scientific reports, 7(1), 13149-13149 (2017-10-17)
Lubricin, a heavily O-glycosylated protein, is essential for boundary lubrication of articular cartilage. Strong surface adherence of lubricin is required given the extreme force it must withstand. Disulfide bound complexes of lubricin and cartilage oligomeric matrix protein (COMP) have recently
Effect of pressure at primary drying of freeze-drying mouse sperm reproduction ability and preservation potential.
Kawase Y et al.
Reproduction (Cambridge, England), 133, 841-841 (2007)
查看所有相关文献

全球贸易项目编号

货号GTIN
H3506-1G04061835557103
H3506-5G04061835557127
H3506-100MG04061835557097
H3506-500MG04061835557110