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Merck
CN

H6648

汉克斯平衡盐 溶液

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

别名:

HBSS

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

shipped in

ambient

storage temp.

room temp

General description

Hanks′平衡盐溶液的基本功能是维持pH值和渗透平衡。它还为细胞提供水和必需的无机离子。
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Application

Hanks平衡盐溶液已被用于:

  • 研究复合材料的降解和细胞毒性作用以及离子和/或磁铁矿从支架中的释放。
  • 测量磁性支架材料在骨组织工程中的稳定性。
  • 细胞内钙离子水平[Ca2+]的荧光测量
  • 从脐带血中分离多形核白细胞(PMN)
  • 评估小鼠的气道炎症

Other Notes

酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于低密度的细胞培养。


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flash_point_c

Not applicable

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

本方案内容:储存——培养的准备工作——培养HDF——传代培养HDF

人心脏成纤维细胞(HCF)分离自成人心室,用于研究心肌梗死、心脏纤维化和心脏肥大。它在细胞外基质维持、生长因子合成和细胞因子合成中起重要作用。本方案概述了人心脏成纤维细胞培养盒传代培养的推荐操作程序。

将细胞放入冻存管后须立即储存在液氮储罐中。

查看所有协议

N Bock et al.
Acta biomaterialia, 6(3), 786-796 (2009-10-01)
In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the
Andreas Koutroulis et al.
Scientific reports, 9(1), 19019-19019 (2019-12-15)
Tricalcium silicate (TCS)-based materials produce calcium hydroxide as a byproduct of their hydration reaction. The present study investigated whether calcium ion release (CIR) affects their biological and antimicrobial properties when used as pulp protection materials. The effect of incorporation of
A Tampieri et al.
Nanotechnology, 22(1), 015104-015104 (2010-12-08)
Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material



全球贸易项目编号

货号GTIN
H6648-1LSTAB04065272480598
H6648-6X500ML04061833801697
H6648-100ML04061833801574
H6648-500ML04061833801604
H6648-6X1L04061833801628
H6648-1L04061833801581