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I9516

2-丙醇

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

别名:

IPA, 仲丙醇, 异丙醇

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关于此项目

线性分子式:
(CH3)2CHOH
化学文摘社编号:
67-63-0
分子量:
60.10
NACRES:
NA.52
PubChem Substance ID:
24896158
UNSPSC Code:
12352104
EC Number:
200-661-7
MDL number:
MFCD00011674
Beilstein/REAXYS Number:
635639
Assay:
≥99.5%
Grade:
Molecular Biology
Technique(s):
DNA extraction: suitable, RNA extraction: suitable
Bp:
82 °C (lit.)
Vapor pressure:
33 mmHg ( 20 °C), 44 mmHg ( 25 °C)
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产品名称

2-丙醇, BioReagent, ≥99.5%, Molecular Biology

grade

Molecular Biology

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

product line

BioReagent

assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable, RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

General description

异丙醇是一种透明、无色、极性的有机溶剂，常用于化学和分子生物学实验室。它可溶解一系列的化物品并快速蒸发。本品（2-丙醇）为分子生物学级，适用于核酸沉淀。

异丙醇使用方案：

  • 从哺乳动物组织沉淀DNA，从DNA样品中提取溴化乙锭，以及从细胞和组织中分离RNA。
  • 蛋白质结晶。
  • 从生物基质中分离类维生素A，进行HPLC和HPLC-MS分析。

Application

2-丙醇已用于：
  • DNA和RNA萃取
  • 测定羟脯氨酸水平（《The Merck Index》第12版）
  • MTT测定检验细胞活力
  • 芯片杂交
适用于使用标准实验流程用于DNA沉淀。还适合作为针对分子生物学应用所制备溶液中的一种溶剂。

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pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Central nervous system

存储类别

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000157104
SHBP3174
SHBP2153
SHBP0616
BCCG6328

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实验方案

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使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程

TRI Reagent® 实验方案

TRI试剂是一种单步RNA分离试剂，可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法，以及各种样品提取方法和故障排除方案。

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

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了解 mRNA 疫苗如何诱导免疫，以及如何为疫苗免疫原和其他生物制药制备合成 mRNA。寻找合成 mRNA 所需的试剂。

SeqPlex™-I Transcriptome Amplification Kits

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

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F Yang et al.
Journal of molecular biology, 288(3), 403-412 (1999-05-18)
The crystal structure of cyanovirin-N (CV-N), a protein with potent antiviral activity, was solved at 1.5 A resolution by molecular replacement using as the search model the solution structure previously determined by NMR. The crystals belong to the space group
Zsuzsanna Rácz et al.
Nucleic acid therapeutics, 21(3), 215-224 (2011-07-14)
Short-interfering RNAs (siRNAs), key mediators of RNA interference comprise a promising therapeutic tool, although side effects such as interferon (IFN) response are still not perfectly understood. Further, delivery to target organs is a major challenge, possibly associated with side effects
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, E-E (1989)
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全球贸易项目编号

货号GTIN
I9516-25ML04061837897221
I9516-1L04061841441250
I9516-4L04061841441267
I9516-4X25ML04061837897238
I9516-500ML04061837897245