form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with Bio-Rad MyiQ, for use with Bio-Rad iCycler iQ, for use with Bio-Rad iQ 5
detection method
SYBR® Green
shipped in
dry ice
storage temp.
−20°C
General description
KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液，含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案，具有最高的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。
高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。
高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。
Application
PCR扩增：
- 基因表达
- DNA定量
- 染色质免疫沉淀(CHiP)
KiCqStart® SYBR® Green qPCR ReadyMix™ has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
Features and Benefits
- 只需33分钟即可获得分析结果
- 高效、灵敏的实时PCR结果
- 几乎不需要多少优化，甚至无需优化
Other Notes
储存条件：
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Legal Information
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
iQ is a trademark of Bio-Rad Laboratories, Inc.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
实验方案
用于测定优化的梯度PCR通过在一系列退火温度下测试含有固定引物浓度的相同反应物来确定引物的最佳退火温度（Ta）。
QPCR 条件的优化对于制定稳健的检测方案非常重要。两种主要优化方法是优化引物浓度和/或退火温度。
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
商品
PCR测定指南可指导您全程了解引物验证和其他测定优化因素，确保实现理想的DNA / RNA定量高灵敏度和特异性。
聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸
传统 PCR 完成后，PCR/qPCR 数据分析通过琼脂糖凝胶或最近的毛细管进行解析。
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 43(12), 1161-1171 (2013-10-26)
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 48, 8-16 (2014-03-04)
The yellow-fever mosquito Aedes aegypti is a major vector of human diseases, such as dengue, yellow fever, chikungunya and West Nile viruses. Chemoreceptor organs on the labella and tarsi are involved in human host evaluation and thus serve as potential
Stephanie Yarwood et al.
Microbial ecology, 69(2), 383-394 (2014-11-06)
The process of pedogenesis and the development of biological communities during primary succession begin on recently exposed mineral surfaces. Following 30 years of surface exposure of reclaimed surface mining sites (Appalachian Mountains, USA), it was hypothesized that microbial communities would
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| KCQS03-SAMPLE | 04061838099495 |
| KCQS03-1250RXN | 04061833945100 |
| KCQS03-250RXN | 04061833945117 |
| KCQS03-5000RXN | 04061833253533 |