M0446
Minimum Essential Medium Eagle, with Earl's salts
StableCell™, with Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
别名:
Eagle最低必需培养基, Minimum Essential Medium Eagle, MEM
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关于此项目
UNSPSC Code:
12352207
NACRES:
NA.75
产品名称
StableCell™ MEM, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): yes
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes
shipped in
ambient
storage temp.
2-8°C
General description
由 Harry Eagle 开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。正常哺乳动物成纤维细胞和某些亚型的 HeLa 细胞的早期尝试培养表明,Eagle 基础培养基（BME）无法满足的他们的特殊营养需求。随后培养这些和其他细胞的研究表明，在 BME 中补充添加剂有利于更多种类的苛求细胞的生长。
MEM 包含了这些改进，包括更高浓度的氨基酸，因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸，扩大了该培养基的用途。
MEM 包含了这些改进，包括更高浓度的氨基酸，因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸，扩大了该培养基的用途。
Application
低限量 Eagle 培养基已用于小鼠成纤维细胞培养，用于体外细胞相容性测试。
Other Notes
该产品不含 L-丙氨酸；L-天冬酰胺；L-谷氨酸；甘氨酸；L-脯氨酸；L-丝氨酸 和 L-谷氨酰胺。该产品补充了 L-Ala-L-Gln 二肽。这为细胞培养提供了更稳定的谷氨酰胺形式。游离氨基酸 L-谷氨酰胺在细胞培养中是不稳定的。
Legal Information
StableCell is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Materials science & engineering. C, Materials for biological applications, 73, 736-742 (2017-02-12)
Effect of processing by equal channel angular pressing (ECAP) on the degradation behaviour of extruded LAE442 magnesium alloy was investigated in a 0.1M NaCl solution, Kirkland's biocorrosion medium (KBM) and Minimum Essential Medium (MEM), both with and without 10% of
Jaroslav Čapek et al.
Materials science & engineering. C, Materials for biological applications, 79, 550-562 (2017-06-21)
Recently, iron-based materials have been considered as candidates for the fabrication of biodegradable load-bearing implants. Alloying with palladium has been found to be a suitable approach to enhance the insufficient corrosion rate of iron-based alloys. In this work, we have
A novel high-strength and highly corrosive biodegradable Fe-Pd alloy: Structural, mechanical and in vitro corrosion and cytotoxicity study
Capek J, et al.
Materials Science and Engineering, C, 79, 550-562 (2017)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M0446-500ML | 04061833993187 |