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M8787

氯化镁 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

别名:

PCR optimization

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关于此项目

线性分子式:
MgCl2
化学文摘社编号:
7786-30-3
分子量:
95.21
NACRES:
NA.52
PubChem Substance ID:
57654422
UNSPSC Code:
12352302
MDL number:
MFCD00011106
Form:
liquid
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Quality Level

200, 300

form

liquid

packaging

vial of 1.5 mL

concentration

25 mM±1 mM

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture
diagnostic assay manufacturing

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

General description

氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加，镁缺乏会导致产量降低。

Application

氯化镁溶液已用作以下材料的组分：

  • 聚合酶链式反应（PCR）扩增混合液
  • 简单序列重复(ISSR)-PCR扩增混合液
  • 16S-23S rRNA基因内转录间隔区（ITS）PCR扩增及限制性内切分析（ITS-PCR- rflp）反应缓冲液
  • 用于定量逆转录-聚合酶链反应（qRT PCR）的PCR反应混合物，以扩增逆转录cDNA
  • 用于RT-PCR检测基因表达的反应混合物
适用于聚合酶链反应的优化。

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCK6176
SLCH7551
SLCC7461
SLBZ9143
SLBQ4119V

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实验方案

通用 SYBR Green qPCR 协议

我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

商品

SeqPlex™-I Transcriptome Amplification Kits

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

Multiplex PCR Assay for Damaged DNA

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

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KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

Optimizing Your Genotyping Workflow
Data Sheet - M8787
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全球贸易项目编号

货号GTIN
M8787-1VL04061834063629
M8787-20RXN04061833297018
M8787-5ML04061838262387
M8787-1.5ML04061838080158
M8787-5VL04061833297025