usage
sufficient for 200 colorimetric tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
activity assay: suitable
input
liquid
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
2-8°C
General description
ATP酶和GTP酶会催化ATP或GTP分解为ADP或GDP和游离磷酸盐。这些酶在运输、信号转导、蛋白质生物合成和细胞分化中起关键作用。
Application
ATPase/GTPase Activity Assay Kit has been used to measure the ATPase enzyme activity in:
- Dicer-like 1 (DCL1) and its mutants, to terminate the reaction and generate a colorimetric product
- various brain regions of mice, facilitating the quantification of inorganic phosphate released from ATP
- cell lysates, for the development and encapsulation of the reconstituted cell-free enzyme system
适于测定ATP酶和GTP酶的活性，也可用于ATP酶和GTP酶抑制剂的筛选试验。
Biochem/physiol Actions
ATPase / GTPase活性检测试剂盒提供了一种简、单直接的以微孔板形式测量ATP酶 / GTP酶活性的方法。该试剂盒使用单一试剂制剂即可在室温下在30分钟内准确测定酶活性。孔雀石绿试剂与酶释放的游离磷酸盐形成稳定的深绿色，产生可在620 nm（600-660 nm）下测定的、与酶活性成正比的比色产物。一个活性单位是在测定条件下每分钟催化产生1μ摩尔游离磷酸盐的酶量。
Features and Benefits
兼容高通量的操作系统。
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
存储类别
12 - Non Combustible Liquids
Shannon E Hill et al.
PloS one, 12(7), e0180241-e0180241 (2017-07-26)
Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because
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Chaoyou Xue et al.
Nucleic acids research, 47(21), 11225-11237 (2019-09-24)
Bloom helicase (BLM) and its orthologs are essential for the maintenance of genome integrity. BLM defects represent the underlying cause of Bloom Syndrome, a rare genetic disorder that is marked by strong cancer predisposition. BLM deficient cells accumulate extensive chromosomal
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAK113-1KT | 04061834065364 |