Quality Level
usage
sufficient for 50 applications (2-5 x 107 cells)
technique(s)
ELISA: suitable, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
General description
线粒体是一种双层膜结构,是真核细胞中大部分能量产生的位点。线粒体分离试剂盒包含两种缓冲液:一种用于提取功能研究的蛋白质,另一种用于蛋白质组学研究的分析。此外,该试剂盒还包括保存完整线粒体的存储缓冲液。
Application
线粒体分离试剂盒用于分离下列来源中的线粒体:
- Ewing′肉瘤肿瘤家族(ESFT)细胞
- 肺结核患者血液样本
- 人神经母细胞瘤细胞和神经胶质瘤细胞
- 肌小管
- HepG2细胞
Features and Benefits
- 该试剂盒允许快速和简单地从细胞中提取浓缩的线粒体部分。
- 分离的线粒体大多具有完整的内外膜。
- 可用于提取线粒体蛋白进行蛋白质组分析
Other Notes
- 本产品仅供研发使用,不用于药物、家庭或其他用途。
- 请查阅材料安全数据表,了解有关危险和安全处理方法的信息。
- 使用超纯水制备试剂。
- 请参阅产品信息表以了解有关试剂盒成分、所需试剂、存储和稳定性以及隔离方案的更多信息。
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Warning
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2
存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
法规信息
低风险生物材料
常规特殊物品
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Shili Duan et al.
The Journal of biological chemistry, 278(2), 1346-1353 (2002-10-31)
We have shown here that the apoptosis inducer staurosporine causes an early decrease in the endogenous respiration rate in intact 143B.TK(-) cells. On the other hand, the activity of cytochrome c oxidase is unchanged for the first 8 h after
The carbonic anhydrase inhibitor methazolamide prevents amyloid beta-induced mitochondrial dysfunction and caspase activation protecting neuronal and glial cells in vitro and in the mouse brain
Fossati S, et al.
Neurobiology of Disease, 86, 29-40 (2016)
D W Moss et al.
The European journal of neuroscience, 13(3), 529-538 (2001-02-13)
Activation of murine microglial and macrophage cell lines with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) resulted in the induction of the inducible form of nitric oxide synthase (NOS) and the release of micromolar amounts of NO into the surrounding medium. The
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| 货号 | GTIN |
|---|---|
| MITOISO2-1KT | 04061834066781 |


