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MOD50

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

别名:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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关于此项目

UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
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Quality Level

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

General description

印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性，使亚硫酸氢盐试剂与单链DNA特异性反应，从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用，包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。

Application

采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。

Features and Benefits

  • 只需要50微微克的DNA或20个细胞
  • 整个流程用时不到2小时
  • 转化率大于99%
  • 降解率极低
  • 便捷一步协议选项
  • 一致且可重复的亚硫酸氢钠转换
  • 可用于基因组、内切核酸酶消化和FFPE DNA

Preparation Note

所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后，溶液可在-20°C下避光保存一周。使用前，必须在室温下解冻冷冻溶液并涡旋2分钟。

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书 & 价格
  • T3566
    Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
    化学品安全说明书

signalword

Danger

supp_hazards

EUH031

存储类别

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

法规信息

危险化学品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCD4557
SLCD4559
SLCF7072
SLCG0978
SLCG0975

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实验方案

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

商品

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

相关内容

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
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全球贸易项目编号

货号GTIN
MOD50-1KT04061834066804