quality
Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum
Quality Level
packaging
pkg of 1 kit
technique(s)
PCR: suitable
application(s)
detection
microbiology
compatibility
Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.
storage temp.
2-8°C
General description
该LookOut®支原体PCR检测试剂盒利用聚合酶链反应（PCR），这是因其在对细胞培养基中的支原体、无胆甾原体和尿素原体以及其他细胞培养衍生的生物制品的检测中具有最高灵敏度而被确定作为选择的方法。该试剂盒已被成功测试具有对20,000至20个拷贝的发酵支原体基因组DNA进行扩增的能力。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法，整体的检测时间得到了大幅度的下降。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法，整体的检测时间得到了大幅度的下降。
Other Notes
该试剂盒已被优化与货号D9307一起使用。不推荐任何带有3’-5’外切核酸酶活性的DNA聚合酶。这些包括Tli、Pfu和Pwo。那些可被使用的DNA聚合酶包括Taq、Tfl和Tth。
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
法规信息
常规特殊物品
此项目有
实验方案
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
商品
通过PCR法、DNA染色法和培养法检测细胞培养中的支原体污染。探索我们的支原体预防、清除和检测试剂盒。
用于细胞系冷冻保存的良好细胞库操作实践概述。
用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MP0035-1KT | 04061834066835 |